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  4. If we were attempt a CAR, cortisol awakening response, hack with salivary cortisol, can you remind us of how to analyze the results? If you have a quick example or even a made-up one, that would be excellent.

If we were attempt a CAR, cortisol awakening response, hack with salivary cortisol, can you remind us of how to analyze the results? If you have a quick example or even a made-up one, that would be excellent.

Chris Kresser: This is an example, including how to interpret it, is on slide number 21 in the DUTCH Test Part 2. I’m not sure exactly what week that was, but basically, with a healthy CAR, you should see a 350 to 600 percent increase in the first 30 minutes. You have the patient—you just order a typical ASI from any lab. Upon rising, the patient rinses his mouth. Have him put a glass of water right next to his bed, and immediately collect his saliva for two to three minutes. Then collect again at 30, 45, and 60 minutes, so that is four samples that you get in the kit. It’s best to drink maybe a little water in between those samples but not eat. He should get out of bed after collecting that first sample, and he should have some exposure to light because that is what activates the suprachiasmatic nucleus and the cortisol awakening response. When you get the results, as I said, you should see a 350 to 600 percent increase in the first 30 minutes no matter what units are used by that particular lab. On slide 21, I have an example of a CAR that was done by Mark Newman, who runs the Precision Analytical and the DUTCH test lab. You’ll see in the chart and the table that he hit 391 percent at 30 minutes, which is within that 350 to 600 percent range.

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